Descrição
The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species.
[This dataset was processed using the GBIF Metabarcoding Data Toolkit.]
Registros de Dados
Os dados deste recurso de ocorrência foram publicados como um Darwin Core Archive (DwC-A), que é o formato padronizado para compartilhamento de dados de biodiversidade como um conjunto de uma ou mais tabelas de dados. A tabela de dados do núcleo contém 2.421 registros.
Também existem 1 tabelas de dados de extensão. Um registro de extensão fornece informações adicionais sobre um registro do núcleo. O número de registros em cada tabela de dados de extensão é ilustrado abaixo.
This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.
Versões
A tabela abaixo mostra apenas versões de recursos que são publicamente acessíveis.
Como citar
Pesquisadores deveriam citar esta obra da seguinte maneira:
Wizenberg S, Zayed A, Pepinelli M, Conflitti I (2024). York University Phenology Project - ITS2, library #2, >0.99 barcode matches. Version 1.1. Naturalis Biodiversity Center. Occurrence dataset. https://ipt.nlbif.nl/resource?r=sbw-yu_pp_its2_l2_99&v=1.1
Direitos
Pesquisadores devem respeitar a seguinte declaração de direitos:
O editor e o detentor dos direitos deste trabalho é Naturalis Biodiversity Center. This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC 4.0) License.
GBIF Registration
Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: 6d5fc2f8-bd5c-40f2-ae10-5b983a2083ec. Naturalis Biodiversity Center publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por Netherlands Biodiversity Information Facility.
Palavras-chave
Occurrence; MaterialSample
Contatos
- Provedor Dos Metadados ●
- Originador ●
- Ponto De Contato
- Postdoctoral Fellow
Cobertura Geográfica
York University, Toronto, Ontario, Canada
| Coordenadas delimitadoras | Sul Oeste [43,775, -79,505], Norte Leste [43,775, -79,505] |
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Cobertura Temporal
| Data Inicial / Data final | 2023-06-07 / 2023-08-16 |
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Dados Sobre o Projeto
Nenhuma descrição disponível
| Título | York University Phenology Project - ITS2, library #2, >0.99 barcode matches |
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Métodos de Amostragem
The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species. [This dataset was processed using the GBIF Metabarcoding Data Toolkit.]
| Área de Estudo | The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species. [This dataset was processed using the GBIF Metabarcoding Data Toolkit.] |
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Descrição dos passos do método:
- The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species. [This dataset was processed using the GBIF Metabarcoding Data Toolkit.]
Metadados Adicionais
| Identificadores alternativos | https://ipt.nlbif.nl/resource?r=sbw-yu_pp_its2_l2_99 |
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