York University Phenology Project - ITS2, library #2, >0.99 barcode matches

Событие
Последняя версия опубликовано Naturalis Biodiversity Center окт. 3, 2024 Naturalis Biodiversity Center
Дата публикации:
3 октября 2024 г.
Опубликовано:
Naturalis Biodiversity Center
Лицензия:
CC-BY-NC 4.0

Скачайте последнюю версию данных этого ресурса в формате Darwin Core Archive (DwC-A) или метаданных ресурса в форматах EML или RTF:

Данные в формате DwC-A Скачать 2 421 Записи в English (137 KB) - Частота обновления: неизвестно
Метаданные в формате EML Скачать в English (13 KB)
Метаданные в формате RTF Скачать в English (11 KB)

Описание

The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species.

[This dataset was processed using the GBIF Metabarcoding Data Toolkit.]

Записи данных

Данные этого occurrence ресурса были опубликованы в виде Darwin Core Archive (DwC-A), который является стандартным форматом для обмена данными о биоразнообразии в виде набора из одной или нескольких таблиц. Основная таблица данных содержит 2 421 записей.

Также в наличии 1 таблиц с данными расширений. Записи расширений содержат дополнительную информацию об основной записи. Число записей в каждой таблице данных расширения показано ниже.

Occurrence (core)
2421
dnaDerivedData 
2421

Данный экземпляр IPT архивирует данные и таким образом служит хранилищем данных. Данные и метаданные ресурсов доступны для скачивания в разделе Загрузки. В таблице версий перечислены другие версии ресурса, которые были доступны публично, что позволяет отслеживать изменения, внесенные в ресурс с течением времени.

Версии

В таблице ниже указаны только опубликованные версии ресурса, которые доступны для свободного скачивания.

Как оформить ссылку

Исследователи должны дать ссылку на эту работу следующим образом:

Wizenberg S, Zayed A, Pepinelli M, Conflitti I (2024). York University Phenology Project - ITS2, library #2, >0.99 barcode matches. Version 1.1. Naturalis Biodiversity Center. Occurrence dataset. https://ipt.nlbif.nl/resource?r=sbw-yu_pp_its2_l2_99&v=1.1

Права

Исследователи должны соблюдать следующие права:

Публикующей организацией и владельцем прав на данную работу является Naturalis Biodiversity Center. Эта работа находится под лицензией Creative Commons Attribution Non Commercial (CC-BY-NC 4.0).

Регистрация в GBIF

Этот ресурс был зарегистрирован в GBIF, ему был присвоен следующий UUID: 6d5fc2f8-bd5c-40f2-ae10-5b983a2083ec.  Naturalis Biodiversity Center отвечает за публикацию этого ресурса, и зарегистрирован в GBIF как издатель данных при оподдержке Netherlands Biodiversity Information Facility.

Ключевые слова

Occurrence; MaterialSample

Контакты

Sydney Wizenberg
  • Metadata Provider
  • Originator
  • Point Of Contact
  • Postdoctoral Fellow
Naturalis Biodiversity Center
Leiden
NL
Amro Zayed
  • Originator
  • Professor
York University
Toronto
Ontario
CA
Mateus Pepinelli
  • Originator
  • Professor
Laurentian University
Sudbury
Ontario
CA
Ida Conflitti
  • Originator
  • Project Manager
York University
Toronto
Ontario
CA

Географический охват

York University, Toronto, Ontario, Canada

Ограничивающие координаты Юг Запад [43,775, -79,505], Север Восток [43,775, -79,505]

Временной охват

Дата начала / Дата окончания 2023-06-07 / 2023-08-16

Данные проекта

Описание отсутсвует

Название York University Phenology Project - ITS2, library #2, >0.99 barcode matches

Методы сбора

The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species. [This dataset was processed using the GBIF Metabarcoding Data Toolkit.]

Охват исследования The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species. [This dataset was processed using the GBIF Metabarcoding Data Toolkit.]

Описание этапа методики:

  1. The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species. [This dataset was processed using the GBIF Metabarcoding Data Toolkit.]

Дополнительные метаданные

Альтернативные идентификаторы https://ipt.nlbif.nl/resource?r=sbw-yu_pp_its2_l2_99