York University Phenology Project - ITS2, library #2, >0.99 barcode matches

Occurrence
Dernière version Publié par Naturalis Biodiversity Center le oct. 3, 2024 Naturalis Biodiversity Center
Date de publication:
3 octobre 2024
Licence:
CC-BY-NC 4.0

Téléchargez la dernière version de la ressource en tant qu'Archive Darwin Core (DwC-A), ou les métadonnées de la ressource au format EML ou RTF :

Données sous forme de fichier DwC-A (zip) télécharger 2 421 enregistrements dans Anglais (137 KB) - Fréquence de mise à jour: inconnue
Métadonnées sous forme de fichier EML télécharger dans Anglais (13 KB)
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Description

The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species.

[This dataset was processed using the GBIF Metabarcoding Data Toolkit.]

Enregistrements de données

Les données de cette ressource occurrence ont été publiées sous forme d'une Archive Darwin Core (Darwin Core Archive ou DwC-A), le format standard pour partager des données de biodiversité en tant qu'ensemble d'un ou plusieurs tableurs de données. Le tableur de données du cœur de standard (core) contient 2 421 enregistrements.

1 tableurs de données d'extension existent également. Un enregistrement d'extension fournit des informations supplémentaires sur un enregistrement du cœur de standard (core). Le nombre d'enregistrements dans chaque tableur de données d'extension est illustré ci-dessous.

Occurrence (noyau)
2421
dnaDerivedData 
2421

Cet IPT archive les données et sert donc de dépôt de données. Les données et métadonnées de la ressource sont disponibles pour téléchargement dans la section téléchargements. Le tableau des versions liste les autres versions de chaque ressource rendues disponibles de façon publique et permet de tracer les modifications apportées à la ressource au fil du temps.

Versions

Le tableau ci-dessous n'affiche que les versions publiées de la ressource accessibles publiquement.

Comment citer

Les chercheurs doivent citer cette ressource comme suit:

Wizenberg S, Zayed A, Pepinelli M, Conflitti I (2024). York University Phenology Project - ITS2, library #2, >0.99 barcode matches. Version 1.1. Naturalis Biodiversity Center. Occurrence dataset. https://ipt.nlbif.nl/resource?r=sbw-yu_pp_its2_l2_99&v=1.1

Droits

Les chercheurs doivent respecter la déclaration de droits suivante:

L’éditeur et détenteur des droits de cette ressource est Naturalis Biodiversity Center. Ce travail est sous licence Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0.

Enregistrement GBIF

Cette ressource a été enregistrée sur le portail GBIF, et possède l'UUID GBIF suivante : 6d5fc2f8-bd5c-40f2-ae10-5b983a2083ec.  Naturalis Biodiversity Center publie cette ressource, et est enregistré dans le GBIF comme éditeur de données avec l'approbation du Netherlands Biodiversity Information Facility.

Mots-clé

Occurrence; MaterialSample

Contacts

Sydney Wizenberg
  • Fournisseur Des Métadonnées
  • Créateur
  • Personne De Contact
  • Postdoctoral Fellow
Naturalis Biodiversity Center
Leiden
NL
Amro Zayed
  • Créateur
  • Professor
York University
Toronto
Ontario
CA
Mateus Pepinelli
  • Créateur
  • Professor
Laurentian University
Sudbury
Ontario
CA
Ida Conflitti
  • Créateur
  • Project Manager
York University
Toronto
Ontario
CA

Couverture géographique

York University, Toronto, Ontario, Canada

Enveloppe géographique Sud Ouest [43,775, -79,505], Nord Est [43,775, -79,505]

Couverture temporelle

Date de début / Date de fin 2023-06-07 / 2023-08-16

Données sur le projet

Pas de description disponible

Titre York University Phenology Project - ITS2, library #2, >0.99 barcode matches

Méthodes d'échantillonnage

The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species. [This dataset was processed using the GBIF Metabarcoding Data Toolkit.]

Etendue de l'étude The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species. [This dataset was processed using the GBIF Metabarcoding Data Toolkit.]

Description des étapes de la méthode:

  1. The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species. [This dataset was processed using the GBIF Metabarcoding Data Toolkit.]

Métadonnées additionnelles