Description
The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species.
[This dataset was processed using the GBIF Metabarcoding Data Toolkit.]
Enregistrements de données
Les données de cette ressource occurrence ont été publiées sous forme d'une Archive Darwin Core (Darwin Core Archive ou DwC-A), le format standard pour partager des données de biodiversité en tant qu'ensemble d'un ou plusieurs tableurs de données. Le tableur de données du cœur de standard (core) contient 2 421 enregistrements.
1 tableurs de données d'extension existent également. Un enregistrement d'extension fournit des informations supplémentaires sur un enregistrement du cœur de standard (core). Le nombre d'enregistrements dans chaque tableur de données d'extension est illustré ci-dessous.
Cet IPT archive les données et sert donc de dépôt de données. Les données et métadonnées de la ressource sont disponibles pour téléchargement dans la section téléchargements. Le tableau des versions liste les autres versions de chaque ressource rendues disponibles de façon publique et permet de tracer les modifications apportées à la ressource au fil du temps.
Versions
Le tableau ci-dessous n'affiche que les versions publiées de la ressource accessibles publiquement.
Comment citer
Les chercheurs doivent citer cette ressource comme suit:
Wizenberg S, Zayed A, Pepinelli M, Conflitti I (2024). York University Phenology Project - ITS2, library #2, >0.99 barcode matches. Version 1.1. Naturalis Biodiversity Center. Occurrence dataset. https://ipt.nlbif.nl/resource?r=sbw-yu_pp_its2_l2_99&v=1.1
Droits
Les chercheurs doivent respecter la déclaration de droits suivante:
L’éditeur et détenteur des droits de cette ressource est Naturalis Biodiversity Center. Ce travail est sous licence Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0.
Enregistrement GBIF
Cette ressource a été enregistrée sur le portail GBIF, et possède l'UUID GBIF suivante : 6d5fc2f8-bd5c-40f2-ae10-5b983a2083ec. Naturalis Biodiversity Center publie cette ressource, et est enregistré dans le GBIF comme éditeur de données avec l'approbation du Netherlands Biodiversity Information Facility.
Mots-clé
Occurrence; MaterialSample
Contacts
- Fournisseur Des Métadonnées ●
- Créateur ●
- Personne De Contact
- Postdoctoral Fellow
Couverture géographique
York University, Toronto, Ontario, Canada
| Enveloppe géographique | Sud Ouest [43,775, -79,505], Nord Est [43,775, -79,505] |
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Couverture temporelle
| Date de début / Date de fin | 2023-06-07 / 2023-08-16 |
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Données sur le projet
Pas de description disponible
| Titre | York University Phenology Project - ITS2, library #2, >0.99 barcode matches |
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Méthodes d'échantillonnage
The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species. [This dataset was processed using the GBIF Metabarcoding Data Toolkit.]
| Etendue de l'étude | The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species. [This dataset was processed using the GBIF Metabarcoding Data Toolkit.] |
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Description des étapes de la méthode:
- The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species. [This dataset was processed using the GBIF Metabarcoding Data Toolkit.]
Métadonnées additionnelles
| Identifiants alternatifs | https://ipt.nlbif.nl/resource?r=sbw-yu_pp_its2_l2_99 |
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