York University Phenology Project - ITS2, library #2, >0.99 barcode matches

オカレンス(観察データと標本)
最新バージョン Naturalis Biodiversity Center により出版 10月 3, 2024 Naturalis Biodiversity Center
公開日:
2024年10月3日
ライセンス:
CC-BY-NC 4.0

DwC-A形式のリソース データまたは EML / RTF 形式のリソース メタデータの最新バージョンをダウンロード:

DwC ファイルとしてのデータ ダウンロード 2,421 レコード English で (137 KB) - 更新頻度: unknown
EML ファイルとしてのメタデータ ダウンロード English で (13 KB)
RTF ファイルとしてのメタデータ ダウンロード English で (11 KB)

説明

The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species.

[This dataset was processed using the GBIF Metabarcoding Data Toolkit.]

データ レコード

この オカレンス(観察データと標本) リソース内のデータは、1 つまたは複数のデータ テーブルとして生物多様性データを共有するための標準化された形式であるダーウィン コア アーカイブ (DwC-A) として公開されています。 コア データ テーブルには、2,421 レコードが含まれています。

拡張データ テーブルは1 件存在しています。拡張レコードは、コアのレコードについての追加情報を提供するものです。 各拡張データ テーブル内のレコード数を以下に示します。

Occurrence (コア)
2421
dnaDerivedData 
2421

この IPT はデータをアーカイブし、データ リポジトリとして機能します。データとリソースのメタデータは、 ダウンロード セクションからダウンロードできます。 バージョン テーブルから公開可能な他のバージョンを閲覧でき、リソースに加えられた変更を知ることができます。

バージョン

次の表は、公にアクセス可能な公開バージョンのリソースのみ表示しています。

引用方法

研究者はこの研究内容を以下のように引用する必要があります。:

Wizenberg S, Zayed A, Pepinelli M, Conflitti I (2024). York University Phenology Project - ITS2, library #2, >0.99 barcode matches. Version 1.1. Naturalis Biodiversity Center. Occurrence dataset. https://ipt.nlbif.nl/resource?r=sbw-yu_pp_its2_l2_99&v=1.1

権利

研究者は権利に関する下記ステートメントを尊重する必要があります。:

パブリッシャーとライセンス保持者権利者は Naturalis Biodiversity Center。 This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC 4.0) License.

GBIF登録

このリソースをはGBIF と登録されており GBIF UUID: 6d5fc2f8-bd5c-40f2-ae10-5b983a2083ecが割り当てられています。   Netherlands Biodiversity Information Facility によって承認されたデータ パブリッシャーとして GBIF に登録されているNaturalis Biodiversity Center が、このリソースをパブリッシュしました。

キーワード

Occurrence; MaterialSample

連絡先

Sydney Wizenberg
  • メタデータ提供者
  • 最初のデータ採集者
  • 連絡先
  • Postdoctoral Fellow
Naturalis Biodiversity Center
Leiden
NL
Amro Zayed
  • 最初のデータ採集者
  • Professor
York University
Toronto
Ontario
CA
Mateus Pepinelli
  • 最初のデータ採集者
  • Professor
Laurentian University
Sudbury
Ontario
CA
Ida Conflitti
  • 最初のデータ採集者
  • Project Manager
York University
Toronto
Ontario
CA

地理的範囲

York University, Toronto, Ontario, Canada

座標(緯度経度) 南 西 [43.775, -79.505], 北 東 [43.775, -79.505]

時間的範囲

開始日 / 終了日 2023-06-07 / 2023-08-16

プロジェクトデータ

説明がありません

タイトル York University Phenology Project - ITS2, library #2, >0.99 barcode matches

収集方法

The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species. [This dataset was processed using the GBIF Metabarcoding Data Toolkit.]

Study Extent The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species. [This dataset was processed using the GBIF Metabarcoding Data Toolkit.]

Method step description:

  1. The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species. [This dataset was processed using the GBIF Metabarcoding Data Toolkit.]

追加のメタデータ