York University Phenology Project - ITS2, library #2, >0.99 barcode matches

Occurrence
最新版本 published by Naturalis Biodiversity Center on 10月 3, 2024 Naturalis Biodiversity Center
發布日期:
2024年10月3日
授權條款:
CC-BY-NC 4.0

下載最新版本的 Darwin Core Archive (DwC-A) 資源,或資源詮釋資料的 EML 或 RTF 文字檔。

DwC-A資料集 下載 2,421 紀錄 在 English 中 (137 KB) - 更新頻率: 有可能更新,但不確知何時
元數據EML檔 下載 在 English 中 (13 KB)
元數據RTF文字檔 下載 在 English 中 (11 KB)

說明

The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species.

[This dataset was processed using the GBIF Metabarcoding Data Toolkit.]

資料紀錄

此資源出現紀錄的資料已發佈為達爾文核心集檔案(DwC-A),其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 2,421 筆紀錄。

亦存在 1 筆延伸集的資料表。延伸集中的紀錄補充核心集中紀錄的額外資訊。 每個延伸集資料表中資料筆數顯示如下。

Occurrence (核心)
2421
dnaDerivedData 
2421

此 IPT 存放資料以提供資料儲存庫服務。資料與資源的詮釋資料可由「下載」單元下載。「版本」表格列出此資源的其它公開版本,以便利追蹤其隨時間的變更。

版本

以下的表格只顯示可公開存取資源的已發布版本。

如何引用

研究者應依照以下指示引用此資源。:

Wizenberg S, Zayed A, Pepinelli M, Conflitti I (2024). York University Phenology Project - ITS2, library #2, >0.99 barcode matches. Version 1.1. Naturalis Biodiversity Center. Occurrence dataset. https://ipt.nlbif.nl/resource?r=sbw-yu_pp_its2_l2_99&v=1.1

權利

研究者應尊重以下權利聲明。:

此資料的發布者及權利單位為 Naturalis Biodiversity Center。 This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC 4.0) License.

GBIF 註冊

此資源已向GBIF註冊,並指定以下之GBIF UUID: 6d5fc2f8-bd5c-40f2-ae10-5b983a2083ec。  Naturalis Biodiversity Center 發佈此資源,並經由Netherlands Biodiversity Information Facility同意向GBIF註冊成為資料發佈者。

關鍵字

Occurrence; MaterialSample

聯絡資訊

Sydney Wizenberg
  • 元數據提供者
  • 出處
  • 連絡人
  • Postdoctoral Fellow
Naturalis Biodiversity Center
Leiden
NL
Amro Zayed
  • 出處
  • Professor
York University
Toronto
Ontario
CA
Mateus Pepinelli
  • 出處
  • Professor
Laurentian University
Sudbury
Ontario
CA
Ida Conflitti
  • 出處
  • Project Manager
York University
Toronto
Ontario
CA

地理涵蓋範圍

York University, Toronto, Ontario, Canada

界定座標範圍 緯度南界 經度西界 [43.775, -79.505], 緯度北界 經度東界 [43.775, -79.505]

時間涵蓋範圍

起始日期 / 結束日期 2023-06-07 / 2023-08-16

計畫資料

無相關描述

計畫名稱 York University Phenology Project - ITS2, library #2, >0.99 barcode matches

取樣方法

The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species. [This dataset was processed using the GBIF Metabarcoding Data Toolkit.]

研究範圍 The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species. [This dataset was processed using the GBIF Metabarcoding Data Toolkit.]

方法步驟描述:

  1. The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species. [This dataset was processed using the GBIF Metabarcoding Data Toolkit.]

額外的詮釋資料